Fernanda cisneros

Handling Editor: David Glover. Nuno M.

Background: In cancer cells, transcriptional gene silencing has been associated with genetic and epigenetic defects. The disruption of DNA methylation patterns and covalent histone marks has been associated with cancer development. In particular, miRb1 has been suggested to be an miRNA with tumor suppressor activity, and it has been shown to be deregulated in various human cancers. In the present study, we evaluated the DNA methylation at the CpG island proximal to the transcription start site of miRb1 in cancer cell lines as well as in normal tissues and gynecological tumor samples. In addition, we analyzed the association of CTCF and covalent histone modifications at the miRb1 locus. CTCF repressive histone marks abundance was evaluated by chromatin immunoprecipitation assays.

Fernanda cisneros

.

Sign In or Create an Account. Published by The Company of Biologists Ltd.

.

Our understanding of the structure and function of mitotic chromosomes has come a long way since these iconic objects were first recognized more than years ago, though many details remain to be elucidated. In this chapter, we start with the early history of chromosome studies and then describe the path that led to our current understanding of the formation and structure of mitotic chromosomes. We also discuss some of the remaining questions. It is now well established that each mitotic chromatid consists of a central organizing region containing a so-called "chromosome scaffold" from which loops of DNA project radially. These proteins are concentrated along the axis of the chromatid.

Fernanda cisneros

J Cell Sci 15 July ; 14 : jcs First Person is a series of interviews with the first authors of a selection of papers published in Journal of Cell Science, helping early-career researchers promote themselves alongside their papers. Nuno conducted the research described in this article while a PhD student in William C. He is now a postdoc in the lab of Ting Wu at Harvard Medical School, Boston, USA, where his research interests lie in the structural and dynamic chromatin regulation of the more mysterious regions of the cell nucleus, such as centromeres, repetitive elements and nucleoli. Fernanda conducted the research described in this article while a postdoc in William C.

Suzume full movie watch online free

In order to investigate the importance of heterochromatin at centromeres, we used epigenetic engineering of a synthetic alphoid tetO human artificial chromosome HAC , to which chimeric proteins can be targeted. Early-career researchers interested in the roles of nuclear lipids, apply now for one of the ten funded places at this Workshop, which will take place October Handling Editor: David Glover. Published by The Company of Biologists Ltd. Nuno M. PDF Link Peer review history. Cite Icon Cite. Email alerts Article activity alert. Fernanda Cisneros-Soberanis The Node preLights FocalPlane. In addition, we analyzed the association of CTCF and covalent histone modifications at the miRb1 locus.

.

Library hub Company news Contacts. Abstract Background: In cancer cells, transcriptional gene silencing has been associated with genetic and epigenetic defects. Background: In cancer cells, transcriptional gene silencing has been associated with genetic and epigenetic defects. In addition, we analyzed the association of CTCF and covalent histone modifications at the miRb1 locus. Conclusions: A reduction of miRb1 expression in cancers, correlated with methylation, repressive histone marks and loss of CTCF binding at the promoter region. All rights reserved. J Cell Sci 14 : jcs Martins Crossref Most eukaryotic centromeres are located within heterochromatic regions. This caused no short-term defects, but long-term tethering reduced HAC centromere protein levels and triggered HAC mis-segregation. Fernanda Cisneros-Soberanis PDF Link Peer review history. Takahiro Nagase , Takahiro Nagase.