Sybr green master mix

Explore high-quality enzymes; now available as individual product s. Explore targets and pathways in their scientific context, find and customize products to study them, analyze data and plan follow-up studies — sybr green master mix in GeneGlobe. High amplification efficiency over a wide dynamic range.

Mixes are also available for fast PCR. The quantity of dsDNA product in the reaction is proportional to the amount of fluorescence. Generally, probes designed for PCR are oligos that have a fluorescent molecule on one end and a quencher on the other. When the oligo primes a PCR reaction and is incorporated into a PCR product, the fluorescent molecule is no longer quenched. Most probe systems require different probes for different sequences.

Sybr green master mix

Yellow sample added to blue master mix yields green qPCR reaction. Visually confirm that your DNA sample was added to master mix. The hot-start enzyme AmpliTaq Gold DNA Polymerase minimizes nonspecific product formation including primer-dimers , yielding superior performance and sensitivity. This minimizes well-to-well variability that can result from a variety of causes, such as pipetting error or sample evaporation. SYBR Green I dye is ideal for target identification screening assays or when a limited number of assays is needed. PCR Equipment and Supplies. Catalog number: Technical Support Customer Service. Catalog Number. Price: , Online price: , Your price: Sign In. Save to list Add to cart. Price: 1. Price: 2.

The corresponding gene may not be expressed above the limit of detection of the qRT-PCR assay method.

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Mixes are also available for fast PCR. The quantity of dsDNA product in the reaction is proportional to the amount of fluorescence. Generally, probes designed for PCR are oligos that have a fluorescent molecule on one end and a quencher on the other. When the oligo primes a PCR reaction and is incorporated into a PCR product, the fluorescent molecule is no longer quenched. Most probe systems require different probes for different sequences. The lack of a requirement for sequence- specific probes can result in shorter experimental preparation times. Many PCR machines require a passive reference dye. An internal reference dye, such as ROX, corrects well-to-well optical variations, and is used for fluorescent signal normalization. This English section is not intended for French healthcare professionals.

Sybr green master mix

View more versions of this product. Catalog No. The Fisher Scientific Encompass Program offers items which are not part of our distribution portfolio.

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Your price: Sign In. Some products have limited regional availability. You may be using too much template. For Research Use Only. Price: 1. In addition, refer to your instrument's user's manual to determine whether the real-time cycler manufacturer recommends use of a plate compression pad during the run. Use less input total RNA for reverse transcription, or use template at a greater dilution factor lower concentration. Another possible explanation pertains to when one is trying to detect cellular expression from an exogenous vector that has been introduced into a cell. Catalog number: The Rn value, or normalized reporter value, is the fluorescent signal from SYBR Green normalized to divided by the signal of the passive reference dye for a given reaction. The lack of a requirement for sequence- specific probes can result in shorter experimental preparation times. There may not have been enough template, in which case, use more input total RNA, or use the template at a lower dilution factor higher concentration , or use a larger volume of template. Order Status Quick Order Support. Browsing Modes for Business Users. What is the threshold cycle or Ct value?

Explore high-quality enzymes; now available as individual product s.

You may be using too much template. The Ct or threshold cycle value is the cycle number at which the fluorescence generated within a reaction crosses the fluorescence threshold, a fluorescent signal significantly above the background fluorescence. PCR Equipment and Supplies. The delta Rn value is the Rn value of an experimental reaction minus the Rn value of the baseline signal generated by the instrument. Use less input total RNA for reverse transcription, or use template at a greater dilution factor lower concentration. There may have been experimental error, in which case, use a template known to contain the gene of interest as a positive control to troubleshoot the PCR reagents and experimental procedure. Events Product News Corporate News. Visually confirm that your DNA sample was added to master mix. There are several reasons for not seeing a PCR product. Price: 1. Many PCR machines require a passive reference dye. Kit Handbooks 1. SYBR Green I dye is ideal for target identification screening assays or when a limited number of assays is needed. Search by lot number or partial lot number.