Spectra viewer

The Spectra viewer tool enables you to visualize the spectral compatibility of fluorophores and fluorescent labelled probes. Revvity Sites Globally Select your location. How to use the SpectraViewer If you're using a Revvity imaging or detection instrument, spectra viewer, start by selecting the instrument from the Select Machine drop-down menu.

Navigation Menu. AAT Bioquest. Cart 0. Sign In. Add a spectrum to begin. Compound Name. How to use this tool AAT Bioquest's interactive Spectrum Viewer is a powerful tool for analysis and comparison of the spectra of common compounds used in absorbance and fluorescence formats.

Spectra viewer

Trusted by leading Companies, Labs and Core Facilities worldwide. Spectra Viewer. FluoroFinder Spectra Viewer is an interactive platform that facilitates fluorescence experiment design. View and compare the spectral properties of more than 1, dyes from all suppliers alongside instrument-specific laser and filter configurations. Spectra Viewer and Experiment Design. A fluorophore with good separation between the excitation and emission maxima results in more reliable detection than a fluorophore with little separation. The continuous development of dyes with improved spectral profiles combined with breakthroughs in light sources, detection methods, and interference filters have paved the way to the adoption of multiplex analysis beyond the realm of flow cytometry. Acquiring large amounts of relevant biological information for each sample has become paramount also in microscopy and imaging experiments. One of the most challenging aspects of multiplex fluorescence analysis is the selection of a combination of fluorochromes with different emission spectra. Spectral overlapping can, in fact, undermine the accuracy and validity of the experiment. It streamlines experiment design and helps to understand which fluorochromes might cause problems, saving troubleshooting time. Pre-loaded light sources and laser sets for microscopy and flow cytometry applications. Laser and filter settings can be manually added to facilitate the selection of fluorophores compatible with the instrument.

Toggling this will normalize the emission spectra to the selected light source line.

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Trusted by leading Companies, Labs and Core Facilities worldwide. Spectra Viewer. FluoroFinder Spectra Viewer is an interactive platform that facilitates fluorescence experiment design. View and compare the spectral properties of more than 1, dyes from all suppliers alongside instrument-specific laser and filter configurations. Spectra Viewer and Experiment Design. A fluorophore with good separation between the excitation and emission maxima results in more reliable detection than a fluorophore with little separation. The continuous development of dyes with improved spectral profiles combined with breakthroughs in light sources, detection methods, and interference filters have paved the way to the adoption of multiplex analysis beyond the realm of flow cytometry. Acquiring large amounts of relevant biological information for each sample has become paramount also in microscopy and imaging experiments.

Spectra viewer

Navigation Menu. AAT Bioquest. Cart 0.

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Trusted by leading Companies, Labs and Core Facilities worldwide. Cart 0. Revvity Sites Globally Select your location. To change the current selection, click a different dye name. Navigation Menu. Sign In. To view a known dye, scroll down the menu on the left and click to select. The user may then "paste" the link into an email or other form of digital communication by either using the right mouse button or clicking the "CTRL" and "v" keys simultaneously. Spectra Viewer and Experiment Design. To predict the response of the fluorophores on the graph to an excitation laser, click "Predict Intensity" under the "Analyze" submenu.

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Under the "Display" submenu heading on the left side of the page, the user can choose to "Hide" or "Show" Fill color within the fluorophore emission graph by clicking the words or left side box. Under Light Source select the light source line which you want to use to normalize the spectra. To change the current selection, click a different excitation source title. FluoroFinder Spectra Viewer is an interactive platform that facilitates fluorescence experiment design. Laser and filter settings can be manually added to facilitate the selection of fluorophores compatible with the instrument. Then enter in the bandwidth number into the new input box and click "OK". These percentages are automatically calculated for each compound currently on the graph. One of the most challenging aspects of multiplex fluorescence analysis is the selection of a combination of fluorochromes with different emission spectra. Toggling this will normalize the emission spectra to the selected light source line. Cart 0. To select a known filter, select one or more options on the left menu. Once all desired options have been highlighted or selected, click the yellow "Add to graph" button on the lower right side.