Smai cut site

Enzymes and Inhibitors. Restriction Enzymes. Catalog number: ER Related applications: Restriction Enzyme Cloning.

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Smai cut site

Hi all, 1 Has anyone used smaI as a RE to cut a vector for blunt end cloning? So my situation now is I'm trying to cut a plasmid bp with smaI. I over digest overnight at room temp. Hope you guys can shed some light SmaI is not the best RE out there, but I understand that sometimes you'd have to use it since it's your only choice. I'd definately phosphatase my plasmid in order to prevent self ligation. I recently used Antarctic Phosphatase and it workes also very good. Good luck!!. Sma I has an isoschizomer which may be a better enzyme. If you still need the blunt end, you can just do a fill-in or chew back the overhang. Hi all, I juz started with cloning in pUC using SmaI to restrict digest for blunt ends and when i ran the gel got a linear plasmid but dint do dephosphorylation

Marker dye for loading DNA samples into gel electrophoresis wells and tracking migration during electrophoresis.

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Smai cut site

This article contains a list of the most studied restriction enzymes whose names start with S. It contains approximately enzymes. The following information is given:. Contents move to sidebar hide. Article Talk. Read Edit View history. Tools Tools.

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Forum Index Home Live Discussion. Select All. There was an error processing your request. This product is available under our Early Access program - Learn More. Preference Cookies We use these cookies to remember your settings and preferences. Failure to heat the water will result in PEG that will take approximately 24 hours to dissolve. This will prevent the Sma I ends of the vector from religating. We and our advertising partners use these cookies to deliver advertisements, to make them more relevant and meaningful to you, and to track the efficiency of our advertising campaigns, both on our services and on other websites and social media. You have 2 remaining attempts. Add 4. Create an Account. SmaI is not the best RE out there, but I understand that sometimes you'd have to use it since it's your only choice. Send Email. Save to list.

Learn more. We are excited to announce that all reaction buffers are now BSA-free.

Minimum of 8 characters Uppercase and lowercase letters At least one number. These advertising cookies will no longer be set for this browser and device. To ensure consistent performance, Thermo Scientific restriction enzyme reaction buffers contain premixed BSA, which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations. Account is invalid. Efficiently ligates DNA inserts into plasmid vectors in just 5—15 minutes at room temperature. You are viewing: R Change Configuration. Username not found. Current Password Incorrect password. United States. Password Password doesn't meet requirements.