N benthamiana

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Federal government websites often end in. The site is secure. Nicotiana benthamiana is widely used as a model plant for dicotyledonous angiosperms. In fact, the strains used in research are highly susceptible to a wide range of viruses. Accordingly, these strains are subject to plant pathology and plant—microbe interactions.

N benthamiana

Data are scarce about the influence of basic cultural conditions on growth patterns and overall performance of plants used as heterologous production hosts for protein pharmaceuticals. Higher plants are complex organisms with young, mature, and senescing organs that show distinct metabolic backgrounds and differ in their ability to sustain foreign protein expression and accumulation. Here, we used the transient protein expression host Nicotiana benthamiana as a model to map the accumulation profile of influenza virus hemagglutinin H1, a clinically promising vaccine antigen, at the whole plant scale. Greenhouse-grown plants submitted to different light regimes, submitted to apical bud pruning, or treated with the axillary growth-promoting cytokinin 6-benzylaminopurine were vacuum-infiltrated with agrobacteria harboring a DNA sequence for H1 and allowed to express the viral antigen for 7 days in growth chamber under similar environmental conditions. Our data highlight the importance of young leaves on H1 yield per plant, unlike older leaves which account for a significant part of the plant biomass but contribute little to total antigen titer. These findings underline the relevance of both considering main stem leaves and axillary stem leaves while modeling heterologous protein production in N. They also demonstrate the potential of exogenously applied growth-promoting hormones to modulate host plant architecture for improvement of protein yields. Several plants are used as heterologous expression hosts to produce recombinant proteins of medical interest, notably including the wild relative of tobacco Nicotiana benthamiana Bally et al. Efficient procedures have been devised for the transient expression of recombinant proteins in N. A variety of therapeutic and diagnostic proteins have been produced in agroinfiltrated N.

Plant-produced recombinant Osteopontin-Fc fusion protein enhanced osteogenesis. Supplementary Table 7C Characteristics and sources of genomes used for comparative analyses.

Nicotiana benthamiana , colloquially known as benth or benthi , is a species of Nicotiana indigenous to Australia. It is a close relative of tobacco. A synonym for this species is Nicotiana suaveolens var. This was transferred to Nicotiana benthamiana by Karel Domin in Bibliotheca Botanica , honoring the original author in the specific epithet. The plant was used by people of Australia as a stimulant, containing nicotine and other alkaloids , before the introduction of commercial tobacco N.

Thank you for visiting nature. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser or turn off compatibility mode in Internet Explorer. In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript. A single lineage of Nicotiana benthamiana is widely used as a model plant 1 and has been instrumental in making revolutionary discoveries about RNA interference RNAi , viral defence and vaccine production. It is peerless in its susceptibility to viruses and its amenability in transiently expressing transgenes 2,3. These unparalleled characteristics have been associated both positively and negatively with a disruptive insertion in the RNA-dependent RNA polymerase 1 gene, Rdr1 4—6. For a plant so routinely used in research, the origin, diversity and evolution of the species, and the basis of its unusual abilities, have been relatively unexplored.

N benthamiana

Nicotiana benthamiana , colloquially known as benth or benthi , is a species of Nicotiana indigenous to Australia. It is a close relative of tobacco. A synonym for this species is Nicotiana suaveolens var. This was transferred to Nicotiana benthamiana by Karel Domin in Bibliotheca Botanica , honoring the original author in the specific epithet. The plant was used by people of Australia as a stimulant, containing nicotine and other alkaloids , before the introduction of commercial tobacco N. Indigenous names for it include tjuntiwari and muntju. The herbaceous plant is found amongst rocks on hills and cliffs throughout the northern regions of Australia. Variable in height and habit, the species may be erect and up to 1. The flowers are white.

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Afterwards, both assemblies were polished with Illumina reads using Pilon 66 v. Effects of plant density on recombinant protein hemagglutinin yields in an Agrobacterium -mediated transient gene expression system using Nicotiana benthamiana plants. Suaveolentes , the section to which N. B A phylogenetic tree constructed among the six plant species. Genome sequencing resulted in 1, scaffolds, including 21 large scaffolds corresponding to the size of the chromosome. Only LTR-transposable elements are shown. PLoS One 7 : e Phylogenetic trees were built using the identified nucleotide sequences and their available counterparts in other Nicotiana species N. Additional studies are now needed to decipher the physiological effects of both cultural practices on H1 yield changes at the plant scale. Nayak, D. Furthermore, some genomic regions and chromosomes are most likely to be lost. Stress activation and genomic impact of Tnt1 retrotransposons in Solanaceae. The methylation ratio of cytosine was calculated as the number of methylated cytosines divided by the number of reads covering that position. Details of the RNA preparation and data preprocessing are described in a previous report Notaguchi et al.

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Effects of CO 2 enrichment, LED inter-lighting, and high plant density on growth of Nicotiana benthamiana used as a host to express influenza virus hemaggulutinin H1. An abundance of recent, intact Copia elements is also evident in N. Nature , 47— Liu, J. Science : — Comparative analysis of N. In particular, low protein content in aging leaves due to reduced synthesis and increased degradation for nitrogen recycling toward growing organs has a strong impact on soluble protein distribution in the plant Avila-Ospina et al. Peer review Peer review information Nature Plants thanks Ed Rybicki, Yongbiao Xue and the other, anonymous, reviewer s for their contribution to the peer review of this work. Therefore, the homologs of the genes between these plants did not clearly correspond to each other Fig. Synteny and orthogroup analyses with other Solanaceae Genome sequence comparisons were performed using D-Genies Cabanettes and Klopp Other studies have described the expression of a viral proton channel to stabilize labile proteins in the cell secretory pathway Jutras et al.