Lycopene beta cyclase

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Carotenoids are important isoprenoids produced in the plastids of photosynthetic organisms that play key roles in photoprotection and antioxidative processes. Xanthi resulted in increased levels of abscisic acid ABA and especially gibberellins GAs , resulting in increased plant yield. In order to understand this phenomenon prior to exporting this genetic strategy to crops, we generated tobacco Nicotiana tabacum cv. Transplastomic plants expressing DcLCYB1 at high levels showed a wild-type-like growth, even though their pigment content was increased and their leaf GA 1 content was reduced. Remarkably, drastic changes in phytohormone content also occurred in the RNAi lines.

Lycopene beta cyclase

However, the roles and underlying mechanisms of the LCYB gene in plant responses to abiotic stresses are rarely known. This gene has not been used to improve carotenoid contents of sweetpotato, Ipomoea batatas L. Shangshu 19 plants. The genes involved in carotenoid and abscisic acid ABA biosynthesis pathways and abiotic stress responses were up-regulated in the transgenic plants. The ABA and proline contents and superoxide dismutase SOD activity were significantly increased, whereas malonaldehyde MDA and H 2 O 2 contents were significantly decreased in the transgenic plants under abiotic stresses. The overall results indicate that the IbLCYB2 gene enhances carotenoid contents and abiotic stress tolerance through positive regulation of carotenoid and ABA biosynthesis pathways in sweetpotato. This gene has the potential to improve carotenoid contents and abiotic stress tolerance in sweetpotato and other plants. Published by Elsevier B. All rights reserved. Substances Carotenoids Intramolecular Lyases lycopene cyclase-isomerase.

Search all BMC articles Search. A rapid LC-MS method for qualitative and quantitative profiling of plant apocarotenoids. Hormone levels were calculated on the basis of the standard isotope dilution method Rittenberg and Foster,

Metrics details. Carotenoids are a group of C40 isoprenoid molecules that play diverse biological and ecological roles in plants. Genes encoding enzymes involved in carotenoid biosynthetic pathway have been cloned. However, regulation of genes involved in carotenoid biosynthetic pathway and accumulation of specific carotenoid in chromoplasts are not well understood. One of the approaches to understand regulation of carotenoid metabolism is to characterize the promoters of genes encoding proteins involved in carotenoid metabolism.

Federal government websites often end in. The site is secure. The composition of carotenoids, along with anthocyanins and chlorophyll, accounts for the distinctive range of colour found in the Actinidia kiwifruit species. Lutein and beta-carotene are the most abundant carotenoids found during fruit development, with beta-carotene concentration increasing rapidly during fruit maturation and ripening. In addition, the accumulation of beta-carotene and lutein is influenced by the temperature at which harvested fruit are stored.

Lycopene beta cyclase

Metrics details. Carotenoids are a group of C40 isoprenoid molecules that play diverse biological and ecological roles in plants. Genes encoding enzymes involved in carotenoid biosynthetic pathway have been cloned. However, regulation of genes involved in carotenoid biosynthetic pathway and accumulation of specific carotenoid in chromoplasts are not well understood. One of the approaches to understand regulation of carotenoid metabolism is to characterize the promoters of genes encoding proteins involved in carotenoid metabolism. To identify promoter region necessary for regulating developmental expression of the ShCYC-B gene, the full-length promoter and its three different 5' truncated fragments were cloned upstream to the initiation codon of GUS reporter cDNA in binary vectors. These four plant transformation vectors were separately transformed in to Agrobacterium. Agrobacterium -mediated transient and stable expression systems were used to study the GUS expression driven by the full-length promoter and its 5' deletion fragments in tomato.

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Constitutive over-expression of chromoplast-specific PSY1 in tomato has been shown to result in dwarf phenotype, probably by interfering with the gibberellin biosynthesis pathway [ 27 ]. Ruf S , Bock R. This indicates ethylene responsive regulation of CYC-B promoter during fruit ripening. Cell , — Samples were evaporated to dryness speed-vac concentrator, Thermo Fisher Scientific without heating. By contrast, at the end of the plant life cycle 3-month-old plants , R2 and R3 plants had a comparable height to wild type plants while R1 remained dwarfed and produced fewer flowers Supplementary Fig. In developing grains, it was interesting that the expression of TaLCYB always remained at a relatively high level, particularly at the later stages. Zeaxanthin has enhanced antioxidant capacity with respect to all other xanthophylls in Arabidopsis leaves and functions independent of binding to PSII antennae. The selected fragments followed the selection strategies for RNAi in wheat [ 53 ]. Figure 7. Carotenoids in the E.

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Diverse roles of strigolactones in plant development. Plant Molecular Biology 62 , — Bock R. A Schematic representation for isoprenoid-derived pathways e. Krinsky NI. Thereby implicating that the basal expression level of CYC-B found in leaves may not have critical role in vegetative tissues. Figure 3. C, D Chloroplasts in the green tissue of R1 leaves are similar to those of the wild type, but differences in starch granule number, apparent size, and shape can be observed. In order to understand how phenotypic changes were present at a microscopic level e. Their provitamin A activity and antioxidant properties are their most attractive functions. This bp promoter region can be used in promoter::reporter fusion molecular genetic screens to identify mutants impaired in CYC-B expression, and thus can be a valuable tool in understanding carotenoid metabolism in tomato. The images of different stages of fruits were derived from one representative line harboring single copy of transgene for each ShCYC-B promoter construct. In A—C the relations of physiological parameters e. In addition, expression analysis were performed following the same parameters and conditions as in Moreno et al. This reduction is in line with the reduced GA, chlorophyll, and carotenoid content.