Luminex assay

Premium instruments, software, calibration, and validation kits to ensure consistent and reproducible results with Luminex xMAP assays.

Attenzione, le pagine di questa sezione sono disponibili solo in lingua inglese. This material is intended for International non-USA site visitors only. Multiplexing is a method for high-volume biomarker testing—or testing multiple analytes simultaneously within a single run—using a single sample volume. Because each microsphere serves as an individual test, a large number of different assays can be performed and analyzed simultaneously. Looking for custom assay design and development services with unique targets in mind?

Luminex assay

Luminex assay is a bead-based immunoassay multiplex technique that allows multiple analytes ex. Generally, the results obtained using a standardized Luminex assay are superior or equivalent to conventional ELISA, even as the differentiated xMAP technology allows high throughput biomarker testing with reduced sample volume, test preparation, and reagent costs. Luminex immunoassays can multiplex up to compatible analytes simultaneously using a commercial or custom kit. Over the past few years, Luminex cytokine assays have been one of our most requested biomarker testing services. Luminex Multiplex utilizes xMAP technology comprising a magnetic or non-magnetic bead-based flow cytometric platform. The Luminex system creates unique spectral addresses for the microspheres by labeling them as per the different ratios of red and far-red fluorophores. This technology can detect multiple targets in a single immunoassay system by assigning each of them with a unique address through the covalent attachment of these fluorescent antibodies to the beads. Thus, this multiplexed immunoassay system with a microplate format can simultaneously analyze up to different nucleic acids, proteins, or peptides. By using the Luminex Multiplex platform, we can detect and quantitate multiple biomarker proteins in the provided samples to evaluate the safety and efficacy of your therapeutics. Furthermore, Luminex bead-based assays can be used in bioanalytical studies utilizing cell-based assays and biomarkers for cardiac, kidney, inflammation, cancer, apoptosis, toxicity, obesity, and intracellular cell signaling including phosphoproteins and nucleic acids. High-performance multiplex assay kits available through this platform can be a valuable asset in basic and translational research on various diseases for the simultaneous analysis of multiple proteins, including growth factors and cytokines such as interferons, interleukins, chemokines, and members of the tumor necrosis superfamily. We partnered on several programs and found NorthEast BioLab responsive, collaborative, and reliable.

Multiplex immunoassays are available for various species and biological matrices such as blood serum, plasma, urine, and lysates. This is the xMAP technology. How luminex assay xMAP Technology work?

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Premium instruments, software, calibration, and validation kits to ensure consistent and reproducible results with Luminex xMAP assays. Validated Luminex xMAP assays for hundreds of analytes: cytokines, chemokines, signaling, and more. Luminex xMap technology is a bead-based multiplexed immunoassay system in a microplate format. The system can simultaneously detect many targets in a single sample — up to , depending on system design. The microspheres used in the Luminex system have different spectral addresses color codes. These unique spectral addresses are created by internally labeling beads with different ratios of two fluorophores, one in a red wavelength and the other in the far red. This is the xMAP technology. Proteins including antibodies, ligands, and nucleic acids specific to the desired targets can be coupled to the beads. Bio-Plex assays use antibodies coupled to the beads. In the kits, every antibody to a different target is covalently attached to beads with a different single spectral address, giving each target its own unique address.

Luminex assay

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The third format is an indirect Luminex assay, where the antigen gets conjugated to the beads and bound by antibodies in the biological sample. How does xMAP Technology work? Some products have limited regional availability. Specialties Back Close Menu. The system can simultaneously detect many targets in a single sample — up to , depending on system design. VP, Development Operations. Our scientists can add extra calibrators or controls at the lower concentrations for increased sensitivity from the multiplex immunoassay. Luminex Assay: Bead-Based Immunoassays Luminex assay is a bead-based immunoassay multiplex technique that allows multiple analytes ex. We trust NorthEast BioLab to design and execute the most streamlined and impactful bioanalytical projects. What types of biological samples can be analyzed on the Luminex Platform? In a Luminex sandwich assay, the analyte binds to a bead conjugated capture antibody and detected by a secondary antibody that contains a fluorophore. We can also increase the number of bead events analyzed for each analyte if a more significant statistical analysis is required than stated in the Luminex multiplex immunoassay protocol. Our scientists can help you readily find Luminex based assay materials and perform appropriate assay development for your biomarkers.

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We can also develop a custom Luminex assay to suit your needs if kits are unavailable commercially. Luminex multiplex assays can analyze biomarkers in multiple biological matrices such as blood serum, plasma, urine, CSF, saliva, synovial fluid, and cell lysates. At NorthEast BioLab , we focus on Luminex assay multiplex development, validation, and sample analysis. We recommend microspheres per analyte in a Luminex multiplex bead assay to obtain highly accurate and precise results. To meet your unique scientific objectives, we sometimes increase the number of events even as most commercial Luminex multiplex assay protocols recommend 50 bead events per analyte per sample. Luminex Assay: High Throughput Multiplex with Strong Sensitivity and Specificity Luminex immunoassays can multiplex by incubating several pre-coated bead sets with different samples in a microtiter plate. How long does a Luminex Assay take? These pages list our product offerings in these areas. We collaborate with numerous qualified vendors to acquire high-quality Luminex assay multiplex kits and reagents for your preclinical and clinical studies. Consequently, Luminex cytokine assays are extensively popular since they can cost-effectively measure various cytokines from minimal sample volume simultaneously. Naturally, more extensive multiplex immunoassay studies may take longer due to the larger number of plate runs. Luminex assay is a bead-based immunoassay multiplex technique that allows multiple analytes ex. If acceptable, the results from study samples get reported to the sponsor in multiple formats. You can create and edit multiple shopping carts. Biomarker Assay List Biomarker research, discovery, and qualification are pivotal in modern drug development from demonstrating proof-of-concept POC to establishing drug safety and efficacy in the clinic….