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These hydroxylated forms of VD3 are useful as pharmaceuticals for the treatment of conditions associated with VD3 deficiency and VD3 metabolic disorder. Herein, we describe the creation of a highly active TA mutant of Vdh by engineering the putative ferredoxin-binding site. Crystallographic and kinetic analyses indicate that the TA mutation results in conformational change from an open to a closed state, thereby increasing the binding affinity with ferredoxin. We also report the efficient biocatalytic synthesis of 25 OH VD3 , a promising intermediate for the synthesis of various hydroxylated VD3 derivatives, by using nisin-treated Rhodococcus erythropolis cells containing VdhTA. The gene-expression cassette encoding Bacillus megaterium glucose dehydrogenase-IV was inserted into the R. A single mutation at the ferredoxin binding site of p vdh enables efficient biocatalytic production of hydroxyvitamin d3. Epub , Sep
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These hydroxylated forms of VD3 are useful as pharmaceuticals for the treatment of conditions associated with VD3 deficiency and VD3 metabolic disorder. Herein, we describe the creation of a highly active TA mutant of Vdh by engineering the putative ferredoxin-binding site. Crystallographic and kinetic analyses indicate that the TA mutation results in conformational change from an open to a closed state, thereby increasing the binding affinity with ferredoxin. We also report the efficient biocatalytic synthesis of 25 OH VD3 , a promising intermediate for the synthesis of various hydroxylated VD3 derivatives, by using nisin-treated Rhodococcus erythropolis cells containing VdhTA. The gene-expression cassette encoding Bacillus megaterium glucose dehydrogenase-IV was inserted into the R. Warning You are using a web browser that we do not support. Our website will not work properly. Please update to a newer version or download a new web browser, such as Chrome or Firefox. Contact us. Data API. This is version 1. A single mutation at the ferredoxin binding site of p vdh enables efficient biocatalytic production of hydroxyvitamin d3. Yasutake, Y. Asymmetric Unit. Macromolecule Content Total Structure Weight:
Diligencia Diligencia is a company that specializes in corporate intelligence and due diligence solutions, 3vrm, operating primarily 3vrm the business services industry. Statement of capital following an allotment of shares on 1 April GBP 1.
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You've rejected analytics cookies. View PDF Confirmation statement made on 31 March with updates - link opens in a new window - 6 pages 6 pages. View PDF Confirmation statement made on 31 March with updates - link opens in a new window - 4 pages 4 pages. Macromolecule Content Total Structure Weight: Warning You are using a web browser that we do not support. You can change your cookie settings at any time. By continuing to use this site you are consenting to these choices. Confirmation statement Next statement date 24 March due by 7 April Last statement dated 24 March Statement of capital following an allotment of shares on 1 April GBP 1. You can read more about your cookie choices at our privacy policy here.
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The company offers a real-time third-party enterprise risk intelligence platform that provides actionable risk insights and continuous monitoring, helping businesses understand and manage the risks associated with their partners, vendors, and suppliers. You've rejected analytics cookies. The company was and is based in London, United Kingdom. Where is 3VRM's headquarters? Accept analytics cookies Reject analytics cookies View cookies. Full crystallographic information is available from OCA. The gene-expression cassette encoding Bacillus megaterium glucose dehydrogenase-IV was inserted into the R. Diligencia primarily serves corporates, financial institutions, government and not-for-profit organizations, and professional services. Cookies on Companies House services We use some essential cookies to make our services work. Companies House does not verify the accuracy of the information filed link opens a new window. We use cookies to make our services work and collect analytics information. Use of these cookies, which may be stored on your device, permits us to improve and customize your experience. CBI websites generally use certain cookies to enable better interactions with our sites and services. View PDF Total exemption full accounts made up to 30 April - link opens in a new window - 10 pages 10 pages. By continuing to use this site you are consenting to these choices.
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